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Isolation of Streptococcus pneumoniae from the sputum samples and their antimicrobial resistance in Biratnagar, Nepal

Journal of Microbiology & Experimentation
Sanjay Mahato,1,2 Hema Kumari Sah,2 Sudhir Yadav2

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Sputum is one of the best specimens for the isolation of respiratory tract infections pathogen. Ten sputum specimens were collected from different hospitals of Biratnagar city where patients of age group 40–73 years suffering from headache, coughing, mild fever, chest pain had visited. In this study, a pathogen of pneumococcal disease was isolated from the sputum sample in 5% goat blood agar by quadrant streak method. The predominant bacterial pathogen isolated from sputum sample was 10% Streptococcus pneumoniae and 90% S. viridian. An old patient of 73 years of age was identified positive with pneumococcal disease with organisms like S. pneumoniae and S. viridian. These pathogens were identified by various biochemical test likewise, catalase, optochin, and bile solubility. S. pneumoniae isolate exhibits high sensitivity against chloramphenicol and amoxicillin but resistance against cefotaxime and erythromycin.


sputum, Streptococcus pneumoniae, S. viridian, Biratnagar, resistance