Genomic sequencing of SARS-CoV-2 samples at the central public health laboratory of Alagoas (Brazil) in June 2023
- International Journal of Molecular Biology: Open Access
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Maria Cidinaria Silva Alves,<sup>1</sup> Jean Phellipe Marques do Nascimento,<sup>2</sup> Mykaella Andrade de Araujo,<sup>3</sup> Magliones Carneiro de Lima,<sup>2</sup> Hazerral de Oliveira Santos,<sup>2</sup> Juliana Vanessa Cavalcante Souza,<sup>2</sup> Anderson Brandão Leite,<sup>2</sup> Sérgio de Sá Leitão Paiva Júnio,<sup>1,4</sup> Valdir de Queiroz Balbino<sup>1</sup>
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Abstract
Genomic surveillance has played a crucial role in monitoring the evolution and spread of SARS-CoV-2, particularly in identifying emerging variants that may influence disease transmission, vaccine efficacy, and therapeutic strategies. In this report, we present the genomic sequencing results of 17 SARS-CoV-2-positive samples collected in Alagoas, Brazil, between May 17 and 29, 2023, and sequenced at the Central Public Health Laboratory of Alagoas (LACEN/AL) from June 5 to 13, 2023. The sequencing was performed using the Illumina MiSeq platform, and lineage classification was conducted using PangoLineages and Nextclade tools. Our analysis identified seven Omicron sublineages: FE.1.2, XBB.1.5, FE.1, FL.4, XBB.1.18.1, XBB.1.5.26, and XBB.1.9.1. The predominance of FE and XBB lineages highlights the ongoing viral evolution and the replacement dynamics of circulating variants. Phylogenetic analysis revealed clustering patterns consistent with global trends, demonstrating the importance of continuous genomic surveillance in tracking SARS- CoV-2 evolution. These findings reinforce the necessity of monitoring emerging variants to inform public health interventions and improve pandemic response strategies. The genomic data generated in this study contribute to a broader understanding of SARS-CoV-2 genetic diversity and its epidemiological impact.
Keywords
SARS-CoV-2, genomic surveillance, variant tracking, omicron lineages, phylogenetic analysis, Brazil
