Water contamination by emerging contaminants is a current problem because these contaminants are not eliminated by conventional treatment methods. Among the emerging contaminants we find hormones such as estradiol, which can bioaccumulate and cause alterations between flora and fauna. In this work, the biodegradation process of the algestone-estradiol hormones was effective by the bacteria Pantoea agglomerans NM1 2.1, Escherichia coli KM4 3.2, and Citrobacter freundii KM1 3.1 in Minimal mineral medium, and Consortium 2, formed by Pantoea agglomerans NM2 1.1, Klebsiella pneumoniae KM2 3.2, Pantoea agglomerans NM1 2.1, and Pantoea agglomerans KM1 2.1, enhanced the biodegradation of the estradiol-algestone hormones compared to the individual strains.