This study aimed to investigate the relationship between lying behaviour and rumination frequency in Holstein breed dairy cows. The study was carried out on a dairy farm with a capacity of 200 milking cows (total of 420 cows)using a continuous observation method
with researchers positioned at four fixed points around the barn. Monthly analyses of rumination frequency in milking cows revealed an average of 60.66 ± 2.32 ruminations in March (range: 37–125), 60.19 ± 2.22 in April (range: 37–82), and 60.19 ± 1.17 in May
(range: 37–125). As a result of the study, the number of ruminations of the standing group was 58,85±2,06 times with a minimum of 37 and a maximum of 125 times, while the number of ruminations of the lying group was 61,33±1,29 times with a minimum of 39,00 and a
maximum of 100,00 times. The differences between the average ruminations of standing and lying cows were not statistically significant. The observed rumination frequencies were within the range considered normal for healthy dairy cows.