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CRISPR construct covering homo sapiens APOL (1-6) genes


MOJ Proteomics & Bioinformatics
Akinseye Olanrewaju Roland,1 Ojomo Joan L,1 Ebenezer Morayo Ale,1 Gbadamosi folawiyo I2

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Abstract

Among the several design tools available for CRISPR/Cas9genome editing, E-CRISP web application was chosen to design single guide RNA (sgRNA) because it provides flexible output, enabling design of multiple libraries. Recently, two coding sequence variants in APOL1, which encodes a trypanolytic factor in humans and gorillas, have been implicated to associate with kidney disease which could result from inaccurate genome editing. There is need to design accurate library of guided RNA for not only APOL1 but also APOL 2-6 for CRISPR/Cas9 genome editing experiment through which one can apply their products in real life experiment.
Method:E-crisp web server was used to design library of single guided RNA (sgRNA) for CRISPR/Cas9 experiments with high specificity, efficacy and annotation covering the Homo sapiens APOL (1-6) genes.
Result:The program was able to design library of single guided RNA (sgRNA) for CRISPR/Cas9 experiments with high specificity, efficacy and annotation covering the Homo sapiens APOL (1-6) genes. APOL6 has highest percentage of library cover with no off-target observed.

Keywords

CRISPR/Cas9, sgRNA, APOL (1-6), library, gene editing, viruses and predators, multilayered defense, sequences in genomes, bloodstream infections, humans and gorillas, killing pathogenic, preliminary step, reference genome, downstream

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