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Preliminary phytochemical, physico-chemical & HPTLC analysis of aanai nerunjil kudineer

International Journal of Complementary & Alternative Medicine
Selvamani S, Justus Antony S, Manoharan A


The Siddha system is an Ancient and Indigineous system of medicine followed predominantly by the people of South India. It comprises of various modes of treatment for a number of diseases. Aanai Nerunjil kudineer is a herbal drug which is indicated for many common Genito-Urinary tract disorders in the textbook, Gunapadam Mooligai vaguppu (Siddha Materia Medica). Aanai Nerunjil (Pedalium murex) plants and Seeds of Kothumalli (Coriandrum sativum) were collected from the locality of Tuticorin, Tamilnadu and were dried under sunshade and ground into coarse powder and then mixed in a 4:1 ratio respectively.

Aqueous alcoholic Extracts of the powder was made by Hot soxhlet method. Alkaloids, Terpenoids, Glycosides were found to be present in preliminary phytochemical investigation. The Physico-chemical analysis revealed that the LOD at 1050c was 8.4%, Total Ash value as 10.84%, acid insoluble ash as 0.85%, water soluble ash as 3.25%, sulphated ash as 14.56%, pH of 4% aqueous solution as 8.08, volatile oil content of 0.5%.HPTLC was performed in aqueous alcoholic extract at 254nm, 366nm & 575nm and the chromatograms were recorded. The Rf peak values were found to be significant which can further assist in identification of the individual compounds.


siddha medicine, pedalium murex, coriandrum sativum, preliminary phytochemical, physico chemical, HPTLC Analysis