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Malaria aptasensor based on electrochemical detection of recombinant plasmodium L dehydrogenase as a biosensor for falsiparium type


Journal of Nanomedicine Research
Laleh Enayati Ahangar, Karim Movassaghi, Fatemeh Yaghoob

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Abstract

A specific aptasensor for a definite type of malaria parasite is created here. This biosensing method is based on electrochemical detection of (Lactate Dehydrogenase) LDH in blood samples. In this research we used a specific Aptamer for recombinant- falsiparium lactate dehydrogenase detection and a specific electrochemical aptasensor was fabricated by glassycarbone electrode (GCE). This biosensor has shown a good performance for accurate detection of rf-pLDH in blood samples, Electrochemical Impedance Spectroscopy (EIS) and amperometric method were used as detection method. NADH oxidation signal was used as chronoamperometric signal as a strategy. The detection 0.8nM and 0.2nM was obtained by EIS and chronoamperometric measurements.

Keywords

electrochemical aptasensor, malaria diagnosis, recombinant plasmodium lactate dehydrogenase, enzymatic detection, chronoamperometry

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