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LC-MS/MS profiling phytochemical content of Echinophora chrysantha (Apiaceae) and antiproliferative, antioxidant activity


Pharmacy & Pharmacology International Journal
Zeynep Aksit,1 Huseyin Aksit,2 Samed Simsek,3 Ali Ayd?n,4 Mustafa Abdullah Yilmaz,5 Ali Kandemir,6 Ekrem Koksal7

Abstract

This study was conducted to determine the antioxidant and antiproliferative activity and phytoconstituents of the hydroalcoholic extract of Echinophora chrysantha (Apiaceae) (EC). LC-MS/MS was used for the identification of non-volatile secondary metabolites. With the LC-MS/MS-based analysis of EC extract, thirty-three non-volatile metabolites were identified belonging to the flavonoid and organic acid categories. The extract showed excellent antiproliferative activity against HT-29 (IC50 =4.07±0.2μg/ml) and HeLa (1.41±0.1μg/ml) with low cytotoxicity toward normal cell lines with IC50 values ranging from 91.24±4.0 to 118.03±3.1μg/ml, inspected through the MTT method. The plant extract showed a high antioxidant activity of IC50=11.52±1.83μg/ml in DPPH and 63.58±4.05µg TE (Trolox equivalent) g-1 in reducing power assay. Identification of metabolites and evaluation of the bioactivity of EC also supports the traditional usage of this plant and its medicinal value. 

Keywords

Echinophora chrysantha, antioxidant, antiproliferative activity

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