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Development of injectable bone substitutes with improved biocompatibility and faster tissue healing for bone tissue defects

Advances in Tissue Engineering & Regenerative Medicine: Open Access
Md Masud Rana,1 Md Arifuzzaman,1 Naznin Akhtar,1 Md Raziul Haque,2 SM Asaduzzaman1

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Tissue engineering is a promising strategy for thereconstruction of bone tissue defects. Calcium phosphate cement (CPC) can be molded or injected to form a scaffold in situ, which imminently conforms to complex bone tissue defects. Here, we have prepared a bone cement with the citric acid-treated human amniotic membrane (HAM), monocalcium phosphate monohydrate (MCPM), and β-tricalcium phosphate (β-TCP). Different physicochemical properties such as setting time, injectability, phase composition, compressive strength, disintegration resistance, biodegradability, bioactivity, in vitro biocompatibility of the bone cement were determined. The bone cement was transplanted in a rabbit long bone defect model to evaluate the new bone formation. The CPC and CPC+HAM both showed the same level of osteo conductivity within long bone defects. At 4 and 12 weeks post-implantation, new bone formation was comparable between the CPC and CPC+HAM based bone substitutes. We conclude that CPC+HAM have excellent potential for use as cement in bone defect reconstruction.


bone cement, human amniotic membrane, monocalcium phosphate monohydrate, ?-TCP, hydroxyapatite, injectability, bioactivity, biocompatibility, long bone defect