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Etched analytical note on various histological staining protocols


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Abstract

In-vitro Microarray method is based on the concept of nucleic acid hybridization which involves comparative analysis of DNA or RNA molecules of identical sequences in the test samples. It also involves various steps of histopathology in a highly sensitive technique, three-dimensional micro-physiological in-vitro cultures, called organoids and spheroids. It is more promising approach for more quick and efficient finding for patient clinical diagnosis relevance with more reliable therapeutic productivity in various case studies. Even so, at present days, more potent and effective characterization methods for three-dimensional structures are controlled in small groups of evaluation of simplistic live/dead techniques. Various protocols proposed for sectioning and staining in-vitro micro tissues which are considered by clinical scientists for examining and visualization of various molecular and biochemical structures like DNA, RNA and protein targets. So that, their respective various morphological examination and immunochemistry staining for in-vitro tissue cultures were performed to test more than hundred numbers of samples at one time or in one go. The cellular or tissue microarray that are explore for high throughput screening and analysis for large number of biological molecules to get more reliable and predictable observation of tissue samples. Microarray involves the evaluation of many similar or same kind of sample having same purpose in a single go rather than performing them individually following their respective immuno histochemistry, immuno fluorescence in situ hybridization.

Keywords

tissue microarray, tissue spheroids, micro physiological system

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