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A study of antimicrobial and antioxidant activity to ethanolic extract of sider (Ziziphus spina-christi) leafs against some Pathogenic microorganisms


Journal of Microbiology & Experimentation
Abdulbaki Al-zaemey, Abdulmageed B Abdullah, Rasheed Al-Husami 

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Abstract

The antimicrobial activity of ethanolic extracts of Ziziphus spina- christi leafs were examined by using agar well diffusion method against five bacteria (Staphylococcus aureus, Pseudomonas aeruginosa, Bacillus cereus, Escherichia coli and Staphylococcus epidermides) in addition to Candida albicans. Results of this present study indicated that the ethanol extract of Ziziphus spina- christi leafs was effective against tested gram positive and negative bacteria whereas the C. albicans was showed resistance to this extract. Results showed that the gram positive bacteria more sensitivity to ethanolic extract of Ziziphus spina- christi leafs at all concentration used (from 5% to 30%) than gram negative bacteria. The highest inhibition zone was by a high concentration of Ziziphus spina-christi ethanolic extract 30% against S. aureus with inhibition zone equal (19mm) while, the lower inhibition zone given by second concentration 20% against P. aeruginosa with inhibition zone equal to (9mm). The results of the 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging activity of ethanolic extract possess high antioxidant activity at different concentrations with IC50 value equal to 176µg ml compared to the IC50 value of control sample (L-Ascorbic acid) equal to 145.88µg/ml.

Keywords

antimicrobial activity, antioxidant activity, Ziziphus spina-christi, ethanolic extract, pathogenic microorganisms

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