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Establishment of an in vivo culture for mycorrhization of Corylus avellana with Tuber melanosporum

Journal of Microbiology & Experimentation
Jorge Aaron Millan Tellez,1 Sayat Ozyilmaz,2 Laura Martinez Montiel

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Tuber melanosporum known as Black Truffle is a mycorrhizal fungus that grows in symbiosis with certain trees and shrubs. Due to its delicate mycorrhization process, the cultivation of this fungus represents a challenge for its large-scale production. The black truffle has a maturation time of 8 to 12 months from its inoculation and is totally dependent on the interaction it carries out with the host plant. Truffle characteristics such as color, odor and flavor can vary depending on the plant with which it is associated, the most commonly used for its cultivation being oaks (Quercus ilex ssp. ilex, Q. ilex ssp. ballota), oaks (Quercus pubescens, Q. cerrioides, Q. petrae, Q. robur), gall oak (Q. faginea), Kermes oak (Q. coccifera), and hazel (Corylus avellana), although associations with other plants have been found in the wild. Similarly, Tuber melanosporum is influenced by the climate in which it grows, the ideal being the Mediterranean climate with hot summers and a high rainfall and winters and autumns with temperatures above -9°C with occasional rainfall. Favorable soils for the cultivation of Tuber melanosporum are limestone soils with clayey, loamy and sandy characteristics, not compacted, which allow the passage and filtration of water, essential for the truffle. Tuber melanosporum has a great value in the gastronomic industry, reaching very high prices depending on its availability, being able to reach prices between 200 and 800 euros per kilogram, however, subsequent processes such as packaging, food preparation and shipping can raise these figures even higher. This project seeks to design a methodology for the in vivo cultivation of Tuber melanosporum, which allows better results in its inoculation and mycorrhization through the use of Tuber melanosporum spores and roots of young hazelnut (Corylus avellana) seedlings, for subsequent transplantation in substrates with the appropriate characteristics for both organisms.


Indole-3-butyric acid, Tuber melanosporum, Mycelium