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Efficiency of purification techniques of human IgG – a review


Journal of Bacteriology & Mycology: Open Access
Zabreen Majeed, Bilqees Naseer, Fatima Iftikhar Shah, Hamid Mukhtar

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Abstract

This review is based on technological trends in IgG purification. Various techniques have been developed to separate antibodies from complex mixtures. Among them are the non-chromatographic and chromatographic techniques. The former consist of techniques such as precipitation, liquid to liquid extraction, and high performance tangential flow filtration. While the latter consist of anion exchange, cation exchange, hydrophobic interaction, immobilized metal affinity, mixed-mode, and bio affinity chromatography. The article also throws light on emerging trend of formulating process buffers to prevent anomalies in the antibodies being purified.

Keywords

IgG purification, chromatography, antibodies, antigen, blood, anion exchange, cation exchange

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