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Scalp psoriasis and biofilms: electron microscopy


Journal of Microbiology & Experimentation
Amalia A Stepanova, Vera G Kornisheva, Konstantin I Raznatovskiy, Olga A Smolina

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Abstract

Psoriasis is an immune-mediated inflammatory skin disease. When examining punch biopsies of a patient’s scalp with psoriasis, we for the first time used scanning electron microscopy (SEM) in combination with the express method of transmission electron microscopy (TEM) - negative contrasting with phosphoric tungsten acid (FVA). The study compared biopsies from scalp affected and unaffected by psoriasis. It was shown that psoriatic areas of the scalp are covered with biofilms consisting of clusters of coccoid bacteria and yeast cells of fungi, connected by thin chaotically located strands. The number of hairs on the affected skin area with psoriasis was 2 times less than on the unaffected one. and the hair cuticle at the lesions was dystrophic. The use of the TEM method revealed only yeast cells in the scalp scales, which, in terms of size, shape (the presence of a wide scar) and monopolar budding, allowed them to be attributed to the genus Malassezia. Fungi of the genus Malassezia were less common in the scales of the affected skin area, as compared to the unaffected one. This discrepancy in the results can be explained by the fact that the formed bacterial-mycotic biofilms, due to the extracellular matrix, affect the vital activity of fungi and prevent them from freely settling between the scales. Most likely, it is the combination of bacteria and fungi in the form of bacterial-mycotic films on the skin that initiates the appearance of psoriatic eruptions, and in further studies it is advisable to consider bacteria and fungi in combination. It is necessary to find out what factors contribute to the formation of such associations during the formation of biofilms, which will allow finding new methods of therapy.

Keywords

biofilms, Malassezia, psoriasis, scalp, scanning and transmission electron microscopy

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