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Polymerase chain reaction: a creative review


Journal of Applied Biotechnology & Bioengineering
Iffat Nazir,1 Hafiz Zaid Mahmood,2 Sana E Mustafa2

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Abstract

In molecular biology, a scientific technique PCR (polymerase chain reaction) is used to generate thousands to millions of copies of a single particular DNA sequences to amplify a single or few copies of a piece of DNA across several orders of magnitude. For multiple applications, PCR is an ordinary and often vital practice used in medicinal and biological research labs and is used for diagnosis and investigation of multiple diseases. In PCR mainly three major steps are involved. Denaturation, annealing, and extension. PCR can be used to detect not only the human genome but also the genome of viruses and bacteria. PCR is especially useful in forensic laborites because a very small amount of original DNA is required. In the development of cancer, genes have been implicated through PCR.

Keywords

polymerase chain, human genome, annealing, extensión

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